Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus.
Identifieur interne : 001931 ( Main/Exploration ); précédent : 001930; suivant : 001932Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus.
Auteurs : Kozue Shimabukuro [Japon] ; Makoto Ujike ; Toshihiro Ito ; Hiroshi Tsunemitsu ; Hitoshi Oshitani ; Fumihiro TaguchiSource :
- Archives of virology [ 1432-8798 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Facteurs de virulence, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- pathogénicité : Torovirus.
- virologie : Érythrocytes.
- Animaux, Attachement viral, Glycoprotéine de spicule des coronavirus, Hémagglutination.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins, Virulence Factors.
- pathogenicity : Torovirus.
- virology : Erythrocytes.
- Animals, Hemagglutination, Spike Glycoprotein, Coronavirus, Virus Attachment.
Abstract
Bovine torovirus (BToV)-Aichi, recently isolated in cultured cells, showed hemagglutination (HA) activity, although the virus has a truncated hemagglutinin-esterase (HE) protein, judging from its gene structure, indicating the existence of another viral protein with HA activity. We examined whether the spike (S) protein possesses HA activity. A BToV antiserum used in this study, reactive to S but not to HE, inhibited HA activity. Furthermore, cells infected with BToV and those expressing S showed hemadsorption (HAD) activity, which was inhibited by the anti-BToV serum; however, HAD activity by expressed HE was not blocked. These data indicate that the S protein of BToV-Aichi is responsible for its HA activity.
DOI: 10.1007/s00705-013-1636-4
PubMed: 23420207
Affiliations:
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viral</term><term>Facteurs de virulence (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Hémagglutination</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Torovirus (pathogénicité)</term><term>Érythrocytes (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de virulence</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Torovirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Torovirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Érythrocytes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Erythrocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Hemagglutination</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Attachment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Attachement viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Hémagglutination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bovine torovirus (BToV)-Aichi, recently isolated in cultured cells, showed hemagglutination (HA) activity, although the virus has a truncated hemagglutinin-esterase (HE) protein, judging from its gene structure, indicating the existence of another viral protein with HA activity. We examined whether the spike (S) protein possesses HA activity. A BToV antiserum used in this study, reactive to S but not to HE, inhibited HA activity. Furthermore, cells infected with BToV and those expressing S showed hemadsorption (HAD) activity, which was inhibited by the anti-BToV serum; however, HAD activity by expressed HE was not blocked. These data indicate that the S protein of BToV-Aichi is responsible for its HA activity. </div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Ito, Toshihiro" sort="Ito, Toshihiro" uniqKey="Ito T" first="Toshihiro" last="Ito">Toshihiro Ito</name><name sortKey="Oshitani, Hitoshi" sort="Oshitani, Hitoshi" uniqKey="Oshitani H" first="Hitoshi" last="Oshitani">Hitoshi Oshitani</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name><name sortKey="Tsunemitsu, Hiroshi" sort="Tsunemitsu, Hiroshi" uniqKey="Tsunemitsu H" first="Hiroshi" last="Tsunemitsu">Hiroshi Tsunemitsu</name><name sortKey="Ujike, Makoto" sort="Ujike, Makoto" uniqKey="Ujike M" first="Makoto" last="Ujike">Makoto Ujike</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Shimabukuro, Kozue" sort="Shimabukuro, Kozue" uniqKey="Shimabukuro K" first="Kozue" last="Shimabukuro">Kozue Shimabukuro</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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